E-ISSN 2577-5669
 

Original Research 


Phenolics-rich extract of guava stem bark inhibits enzymes associated with nephrolithiasis and obesity in vitro

Emmanuel Anyachukwu Irondi.

Abstract
Aim: Inhibition of enzymes catalyzing the production of metabolites that predispose to metabolic diseases is an important index of the potential health benefits of natural products. In this study, the ability of guava (Psidium guajava Linn.) stem bark phenolics-rich extract to inhibit enzymes associated with nephrolithiasis (xanthine oxidase and urease) and obesity [pancreatic lipase and 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase] was evaluated in vitro.
Methods: The phenolics profile of the extract was determined using HPLC-DAD; while the enzymes inhibitory and anti-radical properties of the extract were tested using spectrophotometric methods.
Results: HPLC-DAD fingerprinting revealed that ellagic acid and quercetin were the most abundant phenolic acid and flavonoids, respectively, in the extract. The extract inhibited xanthine oxidase, urease, pancreatic lipase and HMG-CoA reductase, with IC50 values below 40 μg/mL. The extract also exhibited potent anti-radical activity by scavenging ABTS*+, DPPH* and chelating F2+.
Conclusions: Hence, guava stem bark may be a source of nutraceuticals for suppressing the production of uric acid, ammonium hydroxide, fatty acids and cholesterol, and mitigating oxidative stress. These bioactivities, which can be attributed to the phenolic compounds, suggest anti-nephrolithiatic and anti-obesity potentials of guava stem bark phenolics-rich extract.

Key words: Anti-radical; Enzymes inhibition; Guava stem bark; Nephrolithiasis; Obesity; Phenolics


 
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Pubmed Style

Emmanuel Anyachukwu Irondi. Phenolics-rich extract of guava stem bark inhibits enzymes associated with nephrolithiasis and obesity in vitro. J Complement Med Res. 2020; 11(1): 34-42. doi:10.5455/jcmr.2020.11.01.05


Web Style

Emmanuel Anyachukwu Irondi. Phenolics-rich extract of guava stem bark inhibits enzymes associated with nephrolithiasis and obesity in vitro. http://www.jocmr.com/?mno=115985 [Access: June 17, 2020]. doi:10.5455/jcmr.2020.11.01.05


AMA (American Medical Association) Style

Emmanuel Anyachukwu Irondi. Phenolics-rich extract of guava stem bark inhibits enzymes associated with nephrolithiasis and obesity in vitro. J Complement Med Res. 2020; 11(1): 34-42. doi:10.5455/jcmr.2020.11.01.05



Vancouver/ICMJE Style

Emmanuel Anyachukwu Irondi. Phenolics-rich extract of guava stem bark inhibits enzymes associated with nephrolithiasis and obesity in vitro. J Complement Med Res. (2020), [cited June 17, 2020]; 11(1): 34-42. doi:10.5455/jcmr.2020.11.01.05



Harvard Style

Emmanuel Anyachukwu Irondi (2020) Phenolics-rich extract of guava stem bark inhibits enzymes associated with nephrolithiasis and obesity in vitro. J Complement Med Res, 11 (1), 34-42. doi:10.5455/jcmr.2020.11.01.05



Turabian Style

Emmanuel Anyachukwu Irondi. 2020. Phenolics-rich extract of guava stem bark inhibits enzymes associated with nephrolithiasis and obesity in vitro. Journal of Complementary Medicine Research, 11 (1), 34-42. doi:10.5455/jcmr.2020.11.01.05



Chicago Style

Emmanuel Anyachukwu Irondi. "Phenolics-rich extract of guava stem bark inhibits enzymes associated with nephrolithiasis and obesity in vitro." Journal of Complementary Medicine Research 11 (2020), 34-42. doi:10.5455/jcmr.2020.11.01.05



MLA (The Modern Language Association) Style

Emmanuel Anyachukwu Irondi. "Phenolics-rich extract of guava stem bark inhibits enzymes associated with nephrolithiasis and obesity in vitro." Journal of Complementary Medicine Research 11.1 (2020), 34-42. Print. doi:10.5455/jcmr.2020.11.01.05



APA (American Psychological Association) Style

Emmanuel Anyachukwu Irondi (2020) Phenolics-rich extract of guava stem bark inhibits enzymes associated with nephrolithiasis and obesity in vitro. Journal of Complementary Medicine Research, 11 (1), 34-42. doi:10.5455/jcmr.2020.11.01.05