E-ISSN 2577-5669
 

Original Research 


Antifungal effect of eugenol and carvacrol against foodborne pathogens Aspergillus carbonarius and Penicillium roqueforti in improving safety of fresh-cut watermelon

Mirela & 352;imovi& 263;, Frane Dela& 353;, Vedran Gradvol, Dragana Kocevski, Hrvoje Pavlovi& 263;.

Cited by (8)

Abstract
Essential oil components eugenol and carvacrol (ranging between 100 and 200 ppm for carvacrol and between 250 and 750 ppm for eugenol ) were tested for antifungal activity against foodborne pathogenic fungal species A. carbonarius A1102 and P. roqueforti PTFKK29 in vitro and in situ conditions. In vitro antifungal activity of eugenol and carvacrol was evaluated by macrobroth method, while watermelon Citrullus lanatus L. Sorento slices were used for antifungal assays in situ. Selected components, eugenol and carvacrol showed significant inhibitory effect against tested fungi (A. carbonarius A1102 and P.roqueforti PTFKK29) in YES broth, as well as in in situ conditions. The minimal inhibitory concentration (MIC) of eugenol against A. carbonarius A1102 determined by macrobroth method was 2000 ppm, while against P.roqueforti PTFKK29 determined MIC was 1000 ppm. Carvacrol inhibited growth of A. carbonarius A1102 at minimal concentration of 500 ppm, while against P.roqueforti PTFKK29, MIC was 250 ppm. The assays in real food system – watermelon slices for eugenol and carvacrol show that inhibitory effect against both selected fungal species was concentration dependent. Furthermore, our results showed that antifungal effect of carvacrol as well as eugenol applied on watermelon slices in all concentrations was a result of effective synergy between an active antifungal compound and lower incubation temperature (15°C) in inhibition of A. carbonarius A1102. The present study suggests that the use of eugenol and carvacrol is promising natural alternative to the use of food chemical preservatives, in order to improve safety and quality of fresh-cut and ready-to-eat fruits.

Key words: eugenol, carvacrol, Aspergillus carbonarius A1102, Penicillium roqueforti PTFKK29, watermelon, antifungal effect


 
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REFERENCES
1. Mollet B, Rowland I. Functional foods: At the frontier between foodand pharma. Curr Opin Biotechnol 2002;13:483-5. [DOI via Crossref]   
2. de Sousa JP, de Azerêdo GA, de Araújo Torres R, da SilvaVasconcelos MA, da Conceição ML, de Souza EL. Synergies ofcarvacrol and 1, 8-cineole to inhibit bacteria associated with minimallyprocessed vegetables. Int J Food Microbiol 2012;154:145-51. [DOI via Crossref]    [Pubmed]   
3. Gleeson E, Beirne DO. Effects of process severity on survivaland growth of Escherichia coli and Listeria innocua on minimallyprocessed vegetables. Food Control 2005;16:677-85. [DOI via Crossref]   
4. Burt S. Essential oils: Their antibacterial properties and potentialapplications in foods - A review. J Agric Food Chem 2004;94:223-53.
5. Nguefack J, Dongmo JB, Dakole CD, Leth V, Vismer HF, Torp J, et al.Food preservative potential of essential oils and fractions fromCymbopogon citratus, Ocimum gratissimum and Thymus vulgarisagainst mycotoxigenic fungi. Int J Food Microbiol 2009;131:151-6. [DOI via Crossref]    [Pubmed]   
6. Mourey A, Canillac N. Anti-Listeria monocytogenes activity of essentialoils components of conifers. J Agric Food Chem 2002;13:289-92.
7. Carson CF, Riley TV. Safety, efficacy and provenance of tea tree(Melaleuca alternifolia) oil. Contact Dermatitis 2001;45:65-7. [DOI via Crossref]   
8. Lingk W. Health risk evaluation of pesticide contaminations in drinkingwater. Gesunde Pflangen 1991;43:21-5.
9. Lis-Balchin M, Hart S, Deans SG, Eaglesham E. Comparison ofthe pharmacological and antimicrobial action of commercial plantessential oils. J Herbs Spices Med Plants 1996;4:69-86. [DOI via Crossref]   
10. Tolouee M, Alinezhad S, Saberi R, Eslamifar A, Zad SJ, Jaimand K,et al. Effect of Matricaria chamomilla L. flower essential oil on thegrowth and ultrastructure of Aspergillus niger van Tieghem. Int JFood Microbiol 2010;139:127-33. [DOI via Crossref]    [Pubmed]   
11. Bluma R, Amaiden MR, Etcheverry M. Screening of Argentine plantextracts: Impact on growth parameters and aflatoxin B1 accumulationby Aspergillus section Flavi. Int J Food Microbiol 2008;122:114-25. [DOI via Crossref]    [Pubmed]   
12. Unnikrishnan V, Nath BS. Hazardous chemicals in foods. Indian JDairy Biosci 2002;11:155-8.
13. Culter HG, Steverson RF, Cole PD, Jackson DM, Johnson AW.Secondary metabolites from higher plants. Their possible role asbiological control agents. Washington, DC: ACS Symposium Series,American Chemical Society; 1986. p. 178-96.
14. Tripathi P, Dubey NK. Exploitation of natural products as an alternativestrategy to control postharvest fungal rotting of fruit and vegetables.Postharvest Biol Technol 2004;32:235-45. [DOI via Crossref]   
15. Mihaliak CA, Gershenzo J, Croteau R. Lack of rapid monoterpeneturnover in rooted plants, implications for theories of plant chemicaldefense. Oecologia 2006;87:373-6. [DOI via Crossref]   
16. Lin CM, Preston JF 3rd, Wei CI. Antibacterial mechanism of allylisothiocyanate. J Food Prot 2000;63:727-34.
17. Cardile V, Russo A, Formisano C, Rigano D, Senatore F, Arnold NA, et al.Essential oils of Salvia bracteata and Salvia rubifolia from Lebanon:Chemical composition, antimicrobial activity and inhibitory effect onhuman melanoma cells. J Ethnopharmacol 2009;126:265-72. [DOI via Crossref]    [Pubmed]   
18. Pawar VC, Thaker VS. In vitro efficacy of 75 essential oils againstAspergillus niger. Mycoses 2006;49:316-23. [DOI via Crossref]    [Pubmed]   
19. Tajkarimi MM, Ibrahim SA, Cliver DO. Antimicrobial herb and spicecompounds in food. Food Control 2010;21:1199-218. [DOI via Crossref]   
20. Pérez-Alfonso CO, Martínez-Romero D, Zapata PJ, Serrano M,Valero D, Castillo S. The effects of essential oils carvacrol and thymolon growth of Penicillium digitatum and P. Italicum involved in lemondecay. Int J Food Microbiol 2012;158:101-6. [DOI via Crossref]    [Pubmed]   
21. Ultee A, Smid EJ. Influence of carvacrol on growth and toxinproduction by Bacillus cereus. Int J Food Microbiol 2001;64:373-8. [DOI via Crossref]   
22. Pitt JI, Hocking AD. Fungi and Food Spoilage. 3rd ed. Dordrecht:Springer; 2009. p. 237-45.
23. Pati-o B, González-Salgado A, González-Jaén M, Vázquez C.PCR detection assays for the ochratoxin-producing Aspergilluscarbonarius and Aspergillus ochraceus species. Int J Food Microbiol2005;104:207-14.
24. Aydogdu H, Gucer Y. Microfungi and mycotoxins of grapes and grapeproducts. Trakia J Sci 2009;7:211-4.
25. Klich MA. Identification of Common Aspergillus Species. Utrecht:Centraalbureau Voor Schimmelcultures; 2003. p. 46-57.
26. Frisvad JC, Thrane U, Samson RA. Mycotoxin producers. In:Dijksterhuis J, Samson RA, editors. Food Mycology a MultifacetedApproach to Fungi and Food. Boca Raton: CRC Press Taylor & FrancisGroup; 2007. p. 135-63.
27. Suhr KI, Nielsen PV. Antifungal activity of essential oils evaluated bytwo different application techniques against rye bread spoilage fungi.J Appl Microbiol 2003;94:665-74. [DOI via Crossref]   
28. Duraković S, Duraković L. Mikologija u Biotehnologiji. Zagreb: Kugler;2003. p. 213-24.
29. Agarwal SK, Verma S, Singh SS, Tripathi AK, Khan ZK, Kumar S.Antifeedant and antifungal activity of chromene compounds isolatedfrom Blepharispermum subsessile. J Ethnopharmacol 2000;71:231-4. [DOI via Crossref]   
30. Zabka M, Pavela R. Antifungal efficacy of some natural phenoliccompounds against significant pathogenic and toxinogenicfilamentous fungi. Chemosphere 2013;93:1051-6. [DOI via Crossref]    [Pubmed]   

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How to Cite this Article
Pubmed Style

Ma, ia, ,FDa, , . Antifungal effect of eugenol and carvacrol against foodborne pathogens Aspergillus carbonarius and Penicillium roqueforti in improving safety of fresh-cut watermelon. J Complement Med Res. 2014; 3(3): 91-96. doi:10.5455/jice.20140503090524


Web Style

Ma, ia, ,FDa, , . Antifungal effect of eugenol and carvacrol against foodborne pathogens Aspergillus carbonarius and Penicillium roqueforti in improving safety of fresh-cut watermelon. http://www.jocmr.com/?mno=152111 [Access: November 15, 2018]. doi:10.5455/jice.20140503090524


AMA (American Medical Association) Style

Ma, ia, ,FDa, , . Antifungal effect of eugenol and carvacrol against foodborne pathogens Aspergillus carbonarius and Penicillium roqueforti in improving safety of fresh-cut watermelon. J Complement Med Res. 2014; 3(3): 91-96. doi:10.5455/jice.20140503090524



Vancouver/ICMJE Style

Ma, ia, ,FDa, , . Antifungal effect of eugenol and carvacrol against foodborne pathogens Aspergillus carbonarius and Penicillium roqueforti in improving safety of fresh-cut watermelon. J Complement Med Res. (2014), [cited November 15, 2018]; 3(3): 91-96. doi:10.5455/jice.20140503090524



Harvard Style

, M. a. ., , i. a. ., , ,. F. D. a. ., & (2014) Antifungal effect of eugenol and carvacrol against foodborne pathogens Aspergillus carbonarius and Penicillium roqueforti in improving safety of fresh-cut watermelon. J Complement Med Res, 3 (3), 91-96. doi:10.5455/jice.20140503090524



Turabian Style

, Mirela and , imovi and, , Frane Dela and, , Vedran Gradvol, Dragana Kocevski, Hrvoje Pavlovi and, and . 2014. Antifungal effect of eugenol and carvacrol against foodborne pathogens Aspergillus carbonarius and Penicillium roqueforti in improving safety of fresh-cut watermelon. Journal of Complementary Medicine Research, 3 (3), 91-96. doi:10.5455/jice.20140503090524



Chicago Style

, Mirela and , imovi and, , Frane Dela and, , Vedran Gradvol, Dragana Kocevski, Hrvoje Pavlovi and, and . "Antifungal effect of eugenol and carvacrol against foodborne pathogens Aspergillus carbonarius and Penicillium roqueforti in improving safety of fresh-cut watermelon." Journal of Complementary Medicine Research 3 (2014), 91-96. doi:10.5455/jice.20140503090524



MLA (The Modern Language Association) Style

, Mirela and , imovi and, , Frane Dela and, , Vedran Gradvol, Dragana Kocevski, Hrvoje Pavlovi and, and . "Antifungal effect of eugenol and carvacrol against foodborne pathogens Aspergillus carbonarius and Penicillium roqueforti in improving safety of fresh-cut watermelon." Journal of Complementary Medicine Research 3.3 (2014), 91-96. Print. doi:10.5455/jice.20140503090524



APA (American Psychological Association) Style

, M. a. ., , i. a. ., , ,. F. D. a. ., & (2014) Antifungal effect of eugenol and carvacrol against foodborne pathogens Aspergillus carbonarius and Penicillium roqueforti in improving safety of fresh-cut watermelon. Journal of Complementary Medicine Research, 3 (3), 91-96. doi:10.5455/jice.20140503090524





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