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In vitro antimicrobial activity of five essential oils on multi-drug resistant Gram-negative clinical isolates

Hercules Sakkas, Panagiota Gousia, Vangelis Economou, Vassilios Sakkas, Stefanos Petsios, Chrissanthy Papadopoulou.

Cited by (10)

Abstract
Aim/background: The emergence of drug-resistant pathogens has drawn attention on medicinal plants for potential antimicrobial properties. The objective of the present study was the investigation of the antimicrobial activity of five plant essential oils on multi-drug resistant Gram-negative bacteria.
Material and Methods: Basil, Chamomile Blue, Origanum, Thyme and Tea Tree oil were tested against clinical isolates of Acinetobacter baumannii (n=6), Escherichia coli (n=4), Klebsiella pneumoniae (n=7) and Pseudomonas aeruginosa (n=5) using the broth macrodilution method.
Results: The tested essential oils produced variable antibacterial effect, while Chamomile blue oil demonstrated no antibacterial activity. Origanum, Thyme and Basil oils were ineffective on P. aeruginosa isolates. The MIC and MBC values ranged from 0.12 -1.50% (v/v) for Tea Tree oil, 0.25-4% (v/v) for Origanum and Thyme oil, 0.50->4% for Basil oil and >4% for Chamomile Blue oil. Compared to literature data on reference strains the reported MIC values were different by 2SD, denoting less successful antimicrobial activity against multi-drug resistant isolates.
Conclusions: The antimicrobial activities of the essential oils are influenced by the strain origin (wild, reference, drug sensitive or resistant) and it should be taken into consideration whenever investigating the plants’ potential for developing new antimicrobials.

Key words: Essential oil; antimicrobial activity; multi-drug resistant; Klebsiella; Acinetobacter; Escherichia coli; Pseudomonas


 
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How to Cite this Article
Pubmed Style

Hercules Sakkas, Panagiota Gousia, Vangelis Economou, Vassilios Sakkas, Stefanos Petsios, Chrissanthy Papadopoulou. In vitro antimicrobial activity of five essential oils on multi-drug resistant Gram-negative clinical isolates. J Complement Med Res. 2016; 5(3): 212-218. doi:10.5455/jice.20160331064446


Web Style

Hercules Sakkas, Panagiota Gousia, Vangelis Economou, Vassilios Sakkas, Stefanos Petsios, Chrissanthy Papadopoulou. In vitro antimicrobial activity of five essential oils on multi-drug resistant Gram-negative clinical isolates. http://www.jocmr.com/?mno=203358 [Access: November 15, 2018]. doi:10.5455/jice.20160331064446


AMA (American Medical Association) Style

Hercules Sakkas, Panagiota Gousia, Vangelis Economou, Vassilios Sakkas, Stefanos Petsios, Chrissanthy Papadopoulou. In vitro antimicrobial activity of five essential oils on multi-drug resistant Gram-negative clinical isolates. J Complement Med Res. 2016; 5(3): 212-218. doi:10.5455/jice.20160331064446



Vancouver/ICMJE Style

Hercules Sakkas, Panagiota Gousia, Vangelis Economou, Vassilios Sakkas, Stefanos Petsios, Chrissanthy Papadopoulou. In vitro antimicrobial activity of five essential oils on multi-drug resistant Gram-negative clinical isolates. J Complement Med Res. (2016), [cited November 15, 2018]; 5(3): 212-218. doi:10.5455/jice.20160331064446



Harvard Style

Hercules Sakkas, Panagiota Gousia, Vangelis Economou, Vassilios Sakkas, Stefanos Petsios, Chrissanthy Papadopoulou (2016) In vitro antimicrobial activity of five essential oils on multi-drug resistant Gram-negative clinical isolates. J Complement Med Res, 5 (3), 212-218. doi:10.5455/jice.20160331064446



Turabian Style

Hercules Sakkas, Panagiota Gousia, Vangelis Economou, Vassilios Sakkas, Stefanos Petsios, Chrissanthy Papadopoulou. 2016. In vitro antimicrobial activity of five essential oils on multi-drug resistant Gram-negative clinical isolates. Journal of Complementary Medicine Research, 5 (3), 212-218. doi:10.5455/jice.20160331064446



Chicago Style

Hercules Sakkas, Panagiota Gousia, Vangelis Economou, Vassilios Sakkas, Stefanos Petsios, Chrissanthy Papadopoulou. "In vitro antimicrobial activity of five essential oils on multi-drug resistant Gram-negative clinical isolates." Journal of Complementary Medicine Research 5 (2016), 212-218. doi:10.5455/jice.20160331064446



MLA (The Modern Language Association) Style

Hercules Sakkas, Panagiota Gousia, Vangelis Economou, Vassilios Sakkas, Stefanos Petsios, Chrissanthy Papadopoulou. "In vitro antimicrobial activity of five essential oils on multi-drug resistant Gram-negative clinical isolates." Journal of Complementary Medicine Research 5.3 (2016), 212-218. Print. doi:10.5455/jice.20160331064446



APA (American Psychological Association) Style

Hercules Sakkas, Panagiota Gousia, Vangelis Economou, Vassilios Sakkas, Stefanos Petsios, Chrissanthy Papadopoulou (2016) In vitro antimicrobial activity of five essential oils on multi-drug resistant Gram-negative clinical isolates. Journal of Complementary Medicine Research, 5 (3), 212-218. doi:10.5455/jice.20160331064446





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