E-ISSN 2577-5669
 

Research Article 


Design and characterization of Tacrolimus monohydrate loaded core shell lipid polymer hybrid nanoparticle

Hussein Abdelamir Mohammad, Mowafaq M Ghareeb, Mohammd Akrami, Ameer Sabah Sahib.

Abstract
Tacrolimus monohydrate (TAC) loaded core shell lipid polymer hybrid nanoparticle (CS-LPHNs) composed of polymer core (PLGA) surrounded by lipid shell (lecithin). DSPE-PEG2000 was used as stabilize the nanoparticles. TAC-CS-LPHNs were prepared by single step modified nanoprecipitation method. Field emission electron microscope and Transmission Electron Microscope reveals a spherical shape nano-sized particle coated with a lipid layer. The nanoparticles ranged in size from 82nm0.2-1950.143nm which increase with increase the polymer quantity. The entrapment efficiency also affected by the polymer quantity that ranges from 551.23%-761.93%. XRD and DSC studies show conversion of TAC from crystalline into amorphous when imbedded inside the CS-LPHNs and FTIR study shows no chemical interaction between TAC and other components. The Cytotoxicity study of TAC and TAC-CS-LPHNs show a decrease in cytotoxicity of loaded TAC (IC50=1.180670.045g/ml) in compare with free TAC(IC50=0.6970.02g/ml). Finally, the uptake study shows complete internalization of nanoparticle by Vero cell and accumulation of nanoparticles inside nucleus and cytoplasm. The results suggested CS-LPHNs is a good drug delivery system for TAC which is safe, effective and promising a better clinicals outcome.

Key words: lipid polymer hybrid, Tacrolimus monohydrate, IC50, PLGA, Lecithin, LPHNs.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Hussein Abdelamir Mohammad
Articles by Mowafaq M Ghareeb
Articles by Mohammd Akrami
Articles by Ameer Sabah Sahib
on Google
on Google Scholar

How to Cite this Article
Pubmed Style

Hussein Abdelamir Mohammad, Mowafaq M Ghareeb, Mohammd Akrami, Ameer Sabah Sahib. Design and characterization of Tacrolimus monohydrate loaded core shell lipid polymer hybrid nanoparticle. J Complement Med Res. 2020; 11(5): 204-214.


Web Style

Hussein Abdelamir Mohammad, Mowafaq M Ghareeb, Mohammd Akrami, Ameer Sabah Sahib. Design and characterization of Tacrolimus monohydrate loaded core shell lipid polymer hybrid nanoparticle. http://www.jocmr.com/?mno=74069 [Access: June 08, 2021].


AMA (American Medical Association) Style

Hussein Abdelamir Mohammad, Mowafaq M Ghareeb, Mohammd Akrami, Ameer Sabah Sahib. Design and characterization of Tacrolimus monohydrate loaded core shell lipid polymer hybrid nanoparticle. J Complement Med Res. 2020; 11(5): 204-214.



Vancouver/ICMJE Style

Hussein Abdelamir Mohammad, Mowafaq M Ghareeb, Mohammd Akrami, Ameer Sabah Sahib. Design and characterization of Tacrolimus monohydrate loaded core shell lipid polymer hybrid nanoparticle. J Complement Med Res. (2020), [cited June 08, 2021]; 11(5): 204-214.



Harvard Style

Hussein Abdelamir Mohammad, Mowafaq M Ghareeb, Mohammd Akrami, Ameer Sabah Sahib (2020) Design and characterization of Tacrolimus monohydrate loaded core shell lipid polymer hybrid nanoparticle. J Complement Med Res, 11 (5), 204-214.



Turabian Style

Hussein Abdelamir Mohammad, Mowafaq M Ghareeb, Mohammd Akrami, Ameer Sabah Sahib. 2020. Design and characterization of Tacrolimus monohydrate loaded core shell lipid polymer hybrid nanoparticle. Journal of Complementary Medicine Research, 11 (5), 204-214.



Chicago Style

Hussein Abdelamir Mohammad, Mowafaq M Ghareeb, Mohammd Akrami, Ameer Sabah Sahib. "Design and characterization of Tacrolimus monohydrate loaded core shell lipid polymer hybrid nanoparticle." Journal of Complementary Medicine Research 11 (2020), 204-214.



MLA (The Modern Language Association) Style

Hussein Abdelamir Mohammad, Mowafaq M Ghareeb, Mohammd Akrami, Ameer Sabah Sahib. "Design and characterization of Tacrolimus monohydrate loaded core shell lipid polymer hybrid nanoparticle." Journal of Complementary Medicine Research 11.5 (2020), 204-214. Print.



APA (American Psychological Association) Style

Hussein Abdelamir Mohammad, Mowafaq M Ghareeb, Mohammd Akrami, Ameer Sabah Sahib (2020) Design and characterization of Tacrolimus monohydrate loaded core shell lipid polymer hybrid nanoparticle. Journal of Complementary Medicine Research, 11 (5), 204-214.