Pain can be defined as an unpleasant tactile sensation, arousing sensory experience, and an emotional response that is linked to any type of tissue, cell, or skin damage. The pain is a very common and a primary reason for which the people are seeking the health care Among all the non pharmacological methods for pain relieving, the Valsalva maneuver is one of the method which is found effective in reducing the level of pain a person is experiencing during the procedure of intravenous cannulation .This method is easy to perform as well as inexpensive. The procedure of this maneuver is performed by instructing the patient to forceful exhalation after inspiration by closing the nose or against closed airway. The objectives of the study are: To assess the level of pain in patients undergoing peripheral intravenous cannulation in experimental and control group, To assess the effectiveness of Valsalva Maneuver on reducing pain among the patients undergoing peripheral intravenous cannulation between experimental and control group, To determine the association between the post test level of pain in patients undergoing intravenous cannulation with their selected demographic variables. The research design selected for this study was True Experimental ( pre test post test control group design). The total number of samples selected for this study was 60 from which 30 were placed in experimental group and 30 were placed in control group by simple random sampling technique. The setting for this study was Ursala Horsmann Memorial Hospital, Kanpur, Uttar Pradesh. The data was collected by using numerical pain rating scale which scores from 0-10. The result of the study showed that to find the association between post test level of pain with their selected demographic variables, Chi square test was used and it was found that there was a significant association between the level of pain and all their selected demographic variables such as age, gender, body mass index, site of cannulation, number of previous cannulation, size of cannula and previous knowledge regarding Valsalva maneuver. Thus, hypothesis H2 was accepted.To find out the effectiveness of Valsalva maneuver, the paired t-test value was 14.97 for experimental group which is significant at 0.05 level of significance which represents that there is significant difference in pre-test and post-test level of pain in experimental and control group and the value of unpaired t-test was 4.41 which was significant at 0.05 level of significance which represents that the Valsalva maneuver was effective in reducing the level of pain in patients undergoing intravenous cannulation. Thus, hypothesis H1 was accepted.