Shaken baby syndrome is the most common cause of death or serious neurological injury. It is specific to infancy, when children have unique anatomical feature. Subdural and retinal hemorrhages are markers of shaking injury. The present study was conducted to assess the effectiveness of video assisted teaching module on knowledge regarding Shaken Baby Syndrome among mothers of infants in selected rural areas, Kanpur, UP. This is a Quantitative Evaluative Research Approach with a Quasi Experimental One Group Pre-test and Post- test Research Design. The sample were selected by using non-probability convenience sampling technique. The Sample Size was 60 mothers of infants at selected rural areas Kanpur, Uttar Pradesh. The data collection was performed using a Self -Structured Knowledge Questionnaire for interviewing the study participates. The data were analysed using descriptive and inferential statistical analysis. The collected data were analysed by using Descriptive and Inferential statistics. The results of the study represented that Out of 60sample Mothers of infants 53(88.3%) had Inadequate Knowledge, 7(11.6%) had Moderate Knowledge and no one 00(00%) had Adequate Knowledge in Pre-test. Where as in the post test Out 60 Mothers of infants 02(3.3%) had Inadequate Knowledge, 52(86.6%) had Moderate Knowledge and 6(10%) had Adequate Knowledge regarding effects of Shaken Baby Syndrome. Pre -test Mean Knowledge Score 10.75with SD 2.59 and Post -test Knowledge Score 17.33 with SD 1.870 which is less than that of pre-test. Paired “t” test applied to compare Pre-test and Post -test Mean Knowledge Score, and calculated “t” value is 17.95 significant at 0.05 level of significance which indicates that Video Assisted Teaching Module was effective in enhancing the knowledge of Mothers of infants regarding Shaken Baby Syndrome. In the socio -demographic variables there was significant association with the children in the family, occupation of mothers, and previous knowledge and there was no significant association with mothers age, education of mothers, and source of knowledge. Majority of mother had poor knowledge regarding SBS. Because of that new research that has been conducted among mothers of infant the knowledge that has been implemented through video assisted teaching module will improve about the preventive measures & effect of the shaken baby syndrome in mere future.