The present study is to A study to assess the efficacy of video assisted teaching programme regarding HPV Vaccination among adolescent girls in selected college Kanpur u.p. . The objectives of the study are to assess the pre test knowledge score regarding HPV Vaccination among adolescent girls and to assess the effectiveness of video assisted teaching programme regarding HPV vaccination among the adolescent girls and to find out the association between the post test knowledge score with their selected socio demographic variables. The research approach adopted for this study was quantitative evaluative approach. The research design adopted for this study was Quasi - experimental post tests only control group design. The non-probability purposive sampling technique was used in this study. The main aim of this study is to identify the level of knowledge on HPV Infection and its prevention among girls between the age group of 13-18 years. Through evaluative approach pre-experimental design of one group pre-test and post-test design was selected and adopted purposive sampling technique. Tools like structured questionnaire and Video Assisted Teaching on HPV infection and Vaccination its prevention were used. At the first day, pre-test was conducted and Video Assisted Teaching on HPV Infection and its prevention was administered for 20-25 minutes for two batches. In pre-test among the adolescent girls, 36 (60%) had inadequate level of knowledge, 19 (31.67%) had moderate and only 05 (8.33%) had adequate level of knowledge. In post-test among the adolescent girls, 7 (11.67%) had inadequate level of knowledge, 12 (20%) had moderate and 41 (68.33%) had adequate level of knowledge. At the fifth day, post-test was conducted by same tool to assess the effectiveness of Video Assisted Teaching on HPV infection and its prevention.