Nursing is a highly stressful occupation and nurses are particularly at risk for stress related problems, with high rates of turnover. Nursing officers are one of the susceptible groups prone to have sleep disturbances, which may not only influence their own health but also affect the nursing quality and treatment process. Night shift work can disrupt sleep patterns and induce stress. It can disturb the natural rhythmus of cortical and melatonin, which are closely tied to sleep quality. Insufficient sleep is a common complaint among night- shift workers, leading to difficulties in day time sleep after night shifts. The Objective of the study was To assess the quality of sleep among nurses during their clinical night shift duty. To assess the level of stress among nurses during their clinical night shift duty. To determine the correlation between the level of stress and quality of sleep among nurse during their clinical night shift. To find out the association between the level of stress and quality of sleep with selected demographic variables. A cross Sectional descriptive study design was adopted by the researcher among 132 nursing officers at GSVM and Chandni Hospital at Kanpur, U.P. who were selected by using non probability purposive sampling technique. Sleep quality Index and Nursing stress scale was used to collect the data. The study was conducted in GSVM and Chandni hospital Kanpur,U.P. Data collected and analyzed by using descriptive and inferential statistics method.The study result Showed that The descriptive statistics for the Sleep Quality Scale show a mean score of 70.96 with a standard deviation of 15.77, indicating moderate variability around the average sleep quality. The median score is 67.00, suggesting that half of the participants score below this value and half score above. The scores range from a minimum of 46 to a maximum of 112, with a total range of 66 points. The mean percentage of the scores relative to the maximum possible score is 59.1%, indicating that, on average, participants have a moderate level of sleep quality, with room for improvement as the possible gain percentage is 40.9%. The study result show that distribution of nursing stress scores indicates that a significant majority of participants experience a very severe level of stress, with 75.0% scoring between 91 and 120. A smaller proportion, 23.5%, falls into the severe stress category, scoring between 61 and 90. Only 1.5% of participants report a moderate level of stress (31-60), while no participants fall into the mild stress category (0-30). This distribution highlights that most participants are facing high levels of stress. The descriptive statistics for the Nursing StressScale score show that the mean score is 98.73, with a standard deviation of 14.68, indicating variability around the average stress level. The median score is 104.50, suggesting that half of the participants score below this value and half score above.