Foot ulcers are one of the most common and severe complications of diabetes mellitus, with substantial morbidity and mortality. The study displayed the effect of a dipose-derived stem cells (ADSCs)on wound healing in diabetic rats. Diabetes was induced by streptozotocin (STZ) through a single I.P. injection. A full-thickness wound about 10mm in diameter was formed one week after inducing diabetes. Twenty adult male albino rats were divided into Diabetic rats(diabetic with non-treated skin wounds), and stem cellstreated rats (diabetic wounds were treated with a single intradermal injection of 1X106 ADSCs). Fourteen days after making the wound, samples from the wound treated by stem cells revealed a marked increase in the wound closure percentage compared to the diabetic group. The finding was reinforced by histological analysis and electron microscopic study. Also, the gene expression of epidermal growth factor (EGF) and insulin-like growth factor (IGF) were assessed by qRT-PCR. The treated stem cells showed increased EGF and IGF levels compared to the diabetic group. So, the ADSC scan accelerate diabetic wound healing by increasing the growth factors like EGFand IGF.