In research were studyed antagonistic properties of strains of 10 species from the genus Trichoderma isolated from different(soil, plant and water) biotopes of Azerbaijan, and taken to the pure culture against to the phytopathogenic fungi. It became clear that in all the studied strains in relation to cultures such as Alternaria alternata MB-1056, Aspergillus flavus MB-1217, Bipolaris nodulosa MB -2011, Fusarium oxysporum MB-7012, F.solani MB-7034 and Penicillium cyclopium MB11276 which used as a test shows such a feature. It manifests itself at the highest level in the strains Т.asperellum G-3, as well as T.citrinoviride G-26 and Т.koningi G-43. Although there is no correlation between the quantitative indicator of antagonistic activity of fungi from the genus Trichoderma and their cultural morphological type, the strains of T.asperellum G-3 and T.coningi G-43 synthesizes metabolites with strong fungicidal activity, which are considered promising for practical use.