Introduction: Dental caries which is the most common disease of the oral cavity are caused due to demineralization of the inorganic substance and the subsequent destruction of the organic substance of the tooth. They start with the adherence of bacteria to the structures of the tooth, which then will gradually lead to the formation of plaque, culminating in the demineralization of enamel caused by the fermentation of dietary carbohydrates. Mucuna pruriens has long been used as Ayurvedic medicine, as a means to treat Parkinsonism primarily. It is also known to have a variety of other activities which include antiepileptic, antidiabetic, anticariogenic, anti-inflammatory, antioxidative, and also cytotoxic effects. Materials and Methods: 1g Mucuna pruriens was dissolved in distilled water and was boiled for 5 minutes, Titanium dioxide extract was prepared by dissolving the extract in 90ml of distilled water. The mixture was then placed on an orbital shaker for an hour. Result: Maximum activity against all the three oral pathogens were observed at 100 µL. Conclusion: From this study, we can conclude that the high potential of Mucuna pruriens mediated Titanium dioxide nanoparticles is evident on S. mutans and E. faecalis