Introduction: Terminalia arjuna is one of the important herbal plants with cardioprotective, antihyperlipidemic activity and many more applications. In this present investigation, we have prepared aqueous and ethanolic extracts of Terminalia arjuna. Objective: To comparatively evaluate the antioxidant and cytotoxic effect of aqueous and ethanolic extracts of Terminalia arjuna. Methodology: We have studied antioxidant activity of the plant extract by DPPH assay. The cytotoxic effect of prepared Terminalia arjuna plant extract was analyzed using Brine shrimp lethality assay. Results And Conclusion: Regarding the antioxidant activity, both the extracts showed very good activity. However, aqueous extract was more effective at all concentrations. Regarding the cytotoxic effect, only in 2 µL concentration, 80% of napulii were alive for ethanolic extract and 90% of nauplii were alive for aqueous extract. In other concentrations, the results were similar. This proves the less toxic nature of aqueous and ethanolic extracts. Based on the results, we are planning to use aqueous extract of Terminalia arjuna as a local drug delivery agent in the treatment of chronic periodontitis.