Background: Cassia tora is a traditional herbal medicine has been used as an antihyperlipidemic, anti-inflammatory, anti-diabetic, expectorant, antioxidant effect, etc. Objectives: The aim of this study was to evaluate the anxiolytic and antidepressant effect of hydro-alcohol extract of Cassia tora in Swiss mice. Methods: Hydro-alcohol extract of Cassia tora was used for the evaluation of antidepressant, anxiolytic, biochemical estimation, skeletal muscle relaxant, and anoxic tolerance test. Results: The study showed that, in Locomotor activity, there was significant increase in no. of mobility count by Cassia tora extract as compared to negative control and diazepam treatment group (p<0.05, p<0.01). In forced swim test, there was a significant reduction in immobility at different time intervals with 400 mg/kg of Cassia tora extract as compared to negative control and was similar with imipramine treatment group (p<0.01). In a dose of 200 and 400 mg/kg of C. tora, there was a significant reduction in convulsion. At a dose of 400mg/kg, In Elevated Plus Maze test, the mean time spent in open arm increases and in close arm decreases compared to negative control group. Conclusion: Results indicated that Cassia tora extract has antidepressant, anxiolytic and anticonvulsant effect in adult Swiss mice.