Introduction: The copper and selenium nanoparticles (Cu, Se Nps) were synthesized using the aqueous extract of Ficus benghalensis by the green synthesis method. This method of preparation has numerous advantages such as being nontoxic, eco accommodating, less time consuming, and easy to scale up for the synthesis of copper and selenium nanoparticles without incorporating any organic chemicals. Aim: The present study aims at assessing the comparative antimicrobial activity of copper and selenium nanoparticles synthesised using Alampattai against wound pathogens. Materials and Methods: The methodology includes Green synthesis of Alampattai mediated copper and selenium nanoparticles synthesis followed by test for comparative antimicrobial properties against wound pathogens Result: It is evident that Ficus benghalensis mediated copper and seleniumNanoparticles had a significant effect against the pathogens namely S.aureus & E.coli which has the zone of inhibition of about 18 mm and 22 mm respectively which is equivalent to the standard ( Amoxycillin) used. Conclusion: From the study, it is concluded that the both copper and selenium nanoparticles had shown a proportionate increase in antimicrobial activity synthesised using Alampattai with increasing dose concentration.