Aim: Edentulous ridge resorption results in failure of dentures. The aim of this study was to compare the shear and tensile bond strength of reline materials to conventional and 3D printed denture bases. Methods: In this experimental study which was done in 2022, samples(N= 72) devided eight groups.A tensive vertical force was exerted to the end of the samples at 1 mm/min until the tested materials were debonded. For the shear bonding strength, a shear force was exerted to the interface of base and reline material at 1 mm/min using a one-sided shear blade. The force required for detachment of material was recorded . Results: Data showed mean shear bonding strength in conventional groups with TDV reline material and Riviver relining material were obtained 2.22 and 1.89 MPa, while in the 3D printer group, they were 1.34 and 0.81 MPa, respectively. Also tensile bonding strength in the conventional groups with TDV reline material and Riviver reline material were obtained 1.4 and 1.01 MPa, while in the 3D printer group, were obtained 0.64 and 0.45 MPa. Based on paired comparison of groups, they were significantly different (P-value<0.001). Conclusions: The mean tensile and shear bonding strength was higher in all groups fabricated via conventional method as compared to 3D printer method.