Background: Selenium nanoparticles (SeNPs) have the potential to be used for various applications.The green SeNPs (selenium nanoparticles) are formed by simple mixing Withania somnifera (W. somnifera) leaves extract and selenious acid (H2SeO3) solution. Nanoparticles have revolutionized for targeted drug delivery by minimizing the toxicity and side effects that commercially available drugs may have on patients. In this study we have chosen the clove and cumin because it protects from cholesterol, inflammatory damage and it has antioxidant property Aim: The aim of the study is to analyze the antioxidant activity of clove and cumin formulation mediated selenium nanoparticles. Materials & Methods: Cumin and Clove were powdered and stored in an air-sealed container. 1g of cumin and clove formulation was mixed with 100 ml of distilled water and boiled for 15 minutes and the formulation was filtered with Whatmann No.1 filter paper. 30mM sodium selenite was prepared in 50 ml of distilled water. 50 ml of sodium selenite solution and 50 ml of clove and cumin formulation was mixed and kept in a rotary shaker for 48 hours. UV spectroscopy reading based on color change was observed. Results: The antioxidant effect of selenium nanoparticles showed a higher percentage of inhibition at 50µg/ml in DPPH Assay and H2O2 Assay. The clove and cumin extract showed an increased percentage of inhibition with increasing concentration. Conclusion: The clove and cumin formulation mediated selenium nanoparticles acts as potent antioxidant agents. It also has the least side effects compared to commercial drugs.