Aim: To determine antifungal activity of arrow root mediated selenium nanoparticles. Introduction: The field of nanotechnology is one of the most active researches in modern material science. Nanotechnology is emerging as a rapid growing field with its applications in science and technology . It has also been used as the antifungal property and agriculture and anticancer therapy. The production of Se NPs increase the accidental exposure to humans and animals. Materials and Methods: Selenium nanoparticle was prepared using arrow root extract and confirmed by UV-Visible spectrophotometer .The antifungal activity of the synthesized arrow root mediated selenium nanoparticle against Candida albicans was tested by well diffusion technique by standard procedure and the zone of inhibition exhibited by the selenium nanoparticle against the test pathogen the zone of inhibition was measured and graph was made. Result: In the case of 50μL, the zone of inhibition for Candida albicans is 25 mm. This showed that Candida albicans has more activity in 50 μL concentration than standard . In the case of 100μL Candida albicans has the zone of inhibition of 29 mm shows that Candida albicans have the zone of inhibition more than standard. In the case of 150μL the Candida albicans the zone of inhibition is 32 mm. The nanoparticle has a greater zone of inhibition than standard. Conclusion: From this we conclude that arrow root mediated selenium nanoparticle has very good activity against candida albicans which is more than standard antifungal drug. This might be a drug of choice in future.