A new UV spectrophotometric absorption ratio method was developed and validated with force degradation study for simultaneous estimation of pregabalin and mecobalamin in pharmaceutical capsule dosage form. The proposed method is simple, precise, accurate and economic developed and validated according to ICH guidelines. The wavelength (λmax) for detection of pregabalin and mecobalamin were selected as 227nm (isobestic point) and 351nm respectively. The linearity range between 15-90 µg/mL and 0.1-0.9 µg/mL obeys Beer-Lambert’s law with correlation coefficient 0.999 and 0.999 for pregabalin and mecobalamin respectively.The LOD value was 1.845 µg/mL and 0.0166 µg/mL and LOQ value was6.151 µg/mL and 0.0503 µg/mL for pregabalin and mecobalamin respectively. The % assay was found to be 99.65±0.81 % for pregabalin and 99.43±0.66 % for mecobalamin in bulk dosage form. The degradation behavior of the pregabalin and mecobalamin were studied by subjecting to an acid, alkaline, neutral, oxidative, photolytic and thermal condition.