Aim/Background: The indigenous Kuki people have always have a good knowledge of medicinal plants available in their surroundings which are used in treating various illnesses. The present study aims to identify, collect and document traditional uses of ethnomedicinal plants by Thadou-Kuki tribe of Churachandpur district, Manipur. Materials and methods: A total of 80 informants were interviewed through an open-ended and semi-structured questionnaire. The data obtained were analyzed through the Informant Consensus Factor (ICF) and the Fidelity level (FL). Results: It was reported that 55 medicinal plants belonging to 55 genera and 34 families were documented during the survey. The Asteraceae family was the most dominant among all other families. Herbs constituted the largest proportions indigenous to the community with 38.18%. Leaves were the most frequently used plant parts. Decoction and Oral ingestion was found to be the most common mode of preparation and route of application respectively. The consensus analysis revealed that liver diseases, cardiovascular diseases, and respiratory diseases showed the highest ICF with 0.96, 0.92, and 0.91 respectively. While at least four plant species showed 100% FL value namely Centella asiatica, Clerodendrum glandulosum Lindl., Solanum indicum L. and Justicia adhatoda L. used for treating hypertension, and Centella asiatica used for treating gastrointestinal problem also reported 100% FL value. Conclusions: Our data showed that the inhabitants of the studied area still rely on medicinal plants for treating different diseases. Our findings will provide baseline data to exhibit a connection between the traditional knowledge experts and scientific researchers.