Introduction-A root canal treatment's primary goals are the complete elimination of connective tissue, the eradication of any remaining microorganisms discovered in infected root canals, and the creation of a strong seal that will avoid bacterial colonization of the root canal system in the future. Methodology-In the current investigation, 0.16%, 1%, 2.5%, and 5.25% NaOCl solutions, 2% CHX solution and gel, 17% EDTA, 10% citric acid, 37% phosphoric acid, 0.9% saline solution, ethanol, and distilled water were examined. The very same company manufactured all of the chemicals, which were then used right away. Data were analysed using the statistical package SPSS 21 and the QTOF acquisition rate was set to 1.0 second with a 0.4-second inter-scan delay.The studies were performed using a syringe pump at a flow rate of 5.0 mL/min for direct infusion. Results-The relationships' organoleptic qualities. When combined with 1%, 2.5%, and 5.25% NaOCl solutions, 2% CHX gel and solution instantly generated an orange-brown precipitate. When combined with 0.16% NaOCl, it created an orange-white precipitate. A white milky precipitate was noticed right away when CHX was mixed with 17% EDTA. After 5 seconds, a salt precipitation was formed by the associations CHX/saline solution and CHX/ethanol. Conclusion: NaOCl, an oxidising agent that chlorinates the guanidino nitrogens of CHX, is what causes the orange-brown precipitate that is seen in the connection amongst CHX and NaOCl.