Aim: This study was aimed to preliminarily investigate the related-wound biological and wound healing properties of the ethanolic extract of Ya-RakSa-Phlae recipe (E-YRSP) to prove its traditional property. Methods: Phytochemical screening on total phenolics of E-YRSP was observed and GC-MS was also performed to evaluate the chemical profile of the extract. Moreover, biological properties related to wound healing activity such as antibacterial, anti-oxidant, anti-inflammatory, and wound healing activities were carried out. Results: E-YRSP possessed anti-bacterial activity with MIC 16-1,024 μg/mL against both Gram-positive and Gram-negative bacteria. Moreover, it was found to inhibit inflammation with IC50 up to 1,000 μg/mL and oxidation with IC50 ranged from 6-20 μg/mL. It was noted that activities of E-YRSP on anti-infection, antiinflammation, and anti-oxidation markedly resulted in a competent wound healing efficacy. Phenolic compounds were abundantly found in E-YRSP as a large group of phytochemical constituents including catechin, rutin, demethoxycurcumin, luteolin, curcumin, and mangostin which might be a bioactive agent for wound healing promotion. Conclusion: This study has confirmed that YRSP obviously possessed strong wound healing activity as well as other biological properties associated with wound healing including, anti-oxidation, anti-inflammation, and anti-bacteria. Therefore, it is worthwhile to further study on its mechanisms on wound healing process and also to develop as an alternative treatment for wound treatment