Biological methods for nanoparticle synthesis using microorganisms, enzymes, and plants or plant extracts have been suggested as possible eco-friendly alternatives to chemical and physical methods. Bio-mediated synthesis of metal oxide nanoparticles using plant extract is a promising alternative of traditional chemical synthesis. The anti-inflammatory activity of ZnO nanoparticles was done by protein denaturation assay. Finally, the current study has clearly demonstrated that the ZnO NPs are responsible for significant high anti-inflammatory activities. Therefore, the study reveals an efficient, ecofriendly and simple method for the green synthesis of ZnO NPs using green synthetic approach