Aim: The aim of the study is to identify the antibacterial and antifungal activity of Acacia nilotica assisted strontium nanoparticles. Method: Antibacterial activity of respective nanoparticles against the strain Staphylococcus aureus, Bacillus, and E.coli. Mueller Hinton Agar was utilised for this activity to determine the zone of inhibition using Muller Hinton agar