AIM: The aim of the study is to find the antidiabetic activity of piper longum and stevia herbal formulation. INTRODUCTION: Diabetes Mellitus also known as diabetes is a metabolic disorder which is characterised with increased blood glucose level. Frequent urination, increased thirst and increased appetite are the common symptoms. Three types of diabetes are there namely type 1, type 2, and gestational diabetes. Piper longum also known as indian long pepper, belongs to the family piperaceae. It belongs to the indo-malaya region and tropical rainforest region. Stevia rebaudiana commonly known as candly leaf, sweet leaf and sugar leaf belongs to the genus stevia. Stevia is a natural sweetener and natural sugar substitute. MATERIALS AND METHOD: lpha-amylase inhibition was determined by quantifying the amount of maltose liberated during the experiment. Different concentration of nanoparticles (10, 20, 30, 40, 50???????????????????????? ????????s pre-incubated with 100????????L of amylase solution (1U/mL) at room temperature for 30 minutes. DNSA reagent was added to stop the reaction and the solution was heated in a water bath for 5 minutes. Control was maintained where the equal quantity of enzyme extract was replaced by sodium phosphate buffer maintained at a pH value of 6.9. RESULTS AND DISCUSSION: The bar graph represents the antidiabetic activity of piper longum and stevia herbal formulation in various concentrations in ????????????. 10????????L of concentration shows 45% of inhibition, 20????????L of concentration shows 50% of inhibition, 30????????L of concentration shows 65% of inhibition, 40????????L of concentration shows 70% of inhibition and 50????????Lof concentration shows 80% of inhibition. CONCLUSION: From this it is clear that the fruit of piper longum and stevia leaves has proven to be a better choice for antidiabetic activity .