Background: Copper nanoparticles are widely used as antioxidant, antimicrobial , anticancer, anti-inflammatory, antihepatotoxic agents. Being more biocompatible, eliminates the risk for toxicity, ginger has been shown to help reduce blood sugar levels and help regulate insulin response in people. Lemongrass tea is more effective and helps to maintain optimum levels of insulin and improve the glucose tolerance in the body and also helps in preventing the growth of some bacteria and yeast. Aim: The aim of the study is to identify the antimicrobial and cytotoxic effect of lemon grass and ginger formulation assisted copper-oxide nanoparticles Method: Anti-microbial assay is done by testing the sample with certain main microorganisms and for the Cytotoxic effect, Lethal test is done using brine shrimps. Results: Copper nanoparticles synthesized using lemon grass and ginger extract can act as a potential cytotoxic agent and antimicrobial activity. However, the antimicrobial activity of the extract varied with the different bacterial samples. The cytotoxicity of nano- materials and nanocomposites tested against brine shrimps showed minimal lethality. Copper nanoparticles are previously reported to show potent antibacterial and to show cytotoxic activities in-vivo/in-vitro and our results are in accordance with their results. Conclusion: For the first time it is concluded that lemon-grass and ginger mediated Copper nanoparticles are potent therapeutic agents to be used in biomedical applications both in-vivo/in-vitro. Biologically synthesized copper-oxide nanoparticles show exhibitory diverse therapeutic potential which gains more importance currently.