A case-control study include 60 patients with beta-thalassemia major type with recurrent blood transfusion were visited Babylon center of hereditary blood disorder in Babylon teaching hospital for maternity and children and 60 individuals who seemed to be healthy. This study aimed to assess N-acetyl-beta-glucosaminidase and others renal function and to look for renal complications in those patients if any. These patients and control groups divided into two subgroups according to age, group I were less than 18 year and group II were equal or more than 18 years. The present study’s findings showed that there was a significant difference in the levels of Albumin to creatinine ratio, Beta2-microglobulin, N-Acetyl beta-D-glucosaminidase, serum creatinine level, and blood urea between patients group and its healthy group (p < 0.05). In conclusion, beta-thalassemia major patients frequently have renal hemosiderosis and asymptomatic renal impairment, which are not found in routine renal investigations, it needs regular checks for early detection of tubular and glomerular failure.