Background alarms of hemodialysis machine play important global role for patient safety These alarms go off when something is wrong with the machine or threatens patient safety such as blood alarms, dialysate alarms and other alarms. Aim of the study: assess Nurse's Knowledge and Practice level Regarding Hemodialysis Machine Alarms in hemodialysis care unit. Design: A descriptive exploratory research design was used. Setting: The study was conducted in the hemodialysis care units at El-Fayoum General Hospital. Subjects: A Convenient sample of all available nurses (60) nurse from both sex working hemodialysis care units. Tools: two tools were used for data collection. Tool I: Nurses’ self-administered interview Questionnaire: which included (a) Demographic characteristics of nurses (b) Nurses’ knowledge regarding hemodialysis machine alarms Tool II: Observational check list to assess nurses’ practice regarding hemodialysis machine alarms. Results shows that (73.3% and 91.7%) of studied nurses had unsatisfactory level of knowledge and level of practice regarding hemodialysis machine alarm among the studied nurses respectively. Additionally, there was a positive strong high statistically significant correlation between total level of knowledge and total level of practice regarding hemodialysis machine alarm. Conclusion: The study concluded nearly two thirds of the studied nurses had unsatisfactory knowledge regarding hemodialysis machine alarms. And more than three quarter of the studied nurses had incompetent level of practice regarding hemodialysis machine alarms. Additionally, there was a statistically significant correlation between total nurses’ knowledge and practice regarding hemodialysis machine alarms. Recommendations: Continuing educational program to improve knowledge and practices of nurses regarding hemodialysis machine alarms.