Introduction: Surgical site infections are infections that occur after surgery in the body where the surgery took place. They can be superficial or serious, involving tissues under the skin, organs, or implanted material. Surgical site infections contribute to high mortality rates, morbidity, hospitalization length, and treatment costs. Symptoms may be seen as redness, delayed healing, fever, pain, tenderness, warmth, or swelling. Symptoms include pus production from superficial incisions, deep incisions, organ or space SSIs, and abscesses. Methods: From August 2022 to August 2023 in this study. There were 65 pus samples from different surgical wards that were clinically suspected of having post-operative surgical site infections. The MacConkey agar and blood agar media were inoculated using a culture swab, and the medium was then incubated for 24 hours at 37°C. Using a normal microbiological approach, the bacteria from the positive culture were identified after incubation. Staphylococcus aureus strains were identified using the following methods: mannitol fermentation, catalase production test, slide and tube coagulase test, colony morphology, and gram's staining.Antimicrobial susceptibility testing was carried out using the Kirby-Bauer disc diffusion method on Mueller Hinton agar, in compliance with Clinical and Laboratory Standard Institute (CLSI) standards. Results:The current investigation was carried out at Government Medical College Kannauj Following inoculation, a total of 65 samples obtained from clinically suspected post-operative surgical site were examined, 56 (86.15%) had bacterial growth, while 9 (13.85%), exhibited no growth at all following inoculation. In terms of age distribution, the highest rate of post-operative surgical site infection was 57.14%, or 32 out of 65 cases in the 30-40 Age group. This was followed by 19.64%, or 11 cases out of 65 in the 40–50 age group, and the lowest rate was 10.5%, or 7 cases out of 65 in the more than 60 age group. Conclusion: The study carried out between August 2023 to August2024, identified 56 bacterial growth isolates from post-operative surgical site infection. Worldwide, there is a genuine danger of postoperative wound infections with all surgical procedures and medical treatments. It is one of the main causes of much higher rates of morbidity, death, unwarranted longer hospital stays, and increased medical expenses for surgical patients. Every hospital should conduct periodic surveillance of postoperative wound infections at regular intervals in order to evolve control strategies and lower the infection rate, as the control of post-operative complications is a crucial aspect of overall management.