Introduction: The major components present in ginger are alpha-zingiberene, gingerols and shogaols. Ginger oleoresin are used as aromatic, carminative, stomachic and stimulant. Zinc oxide nanoparticles have a diameter less than 100 nm. Zinc oxide nanoparticles are used due to its biosensor, cosmetics, storage, optical devices, window materials for displays, solar cells, and drug-delivery properties. Materials And Methods: Ginger oleoresin was purchased and extract was prepared by dissolving in distilled water. Zinc oxide was added and it was characterised by UV visible spectroscopy method. Antibacterial and antifungal potential of the extract assessed by agar well diffusion method. Results: Zinc oxide nanoparticles synthesised using ginger oleoresin shows antimicrobial activity. Dose dependent antimicrobial activity was observed. The prepared extract showed the highest zone of inhibition , 25 mm at 100μl against S.aureus and E.faecalis showed a maximum zone of inhibition of 24 mm at 100μl . Conclusion: Ginger oleoresin mediated zinc oxide nanoparticles showed a potent antimicrobial activity against C.albicans, S.mutans, S.aureus and E.faecalis. Hence it may be formulated to treat infections caused by such organisms after further studies