Introduction: Indian spice contributes to food as both aroma and taste. Indian spices have been shown to treat diseases that range from colds, cough, and cancer. Ginger is a member of the Zingiberaceae genus, scientifically known as Zingiber officinale. It is the plant with medicinal and nutritional qualities that is the most essential. Aim: The present study was conducted to evaluate the cytotoxic effect of ginger oleoresin against A549 lung cancer cell line. Materials and Method: The Ginger oleoresin extract is collected from Synthetic Industries, with a product code - 4010000370. MTT assay was performed on the A549 cell line to assess the cytotoxic effect. The A549 cells are treated with different concentrations of ginger oleoresin (10, 20, 30, 50, 100 and 200 μg/ml) for 24 hrs and the cell viability was analysed using MTT assay. Results: The results of MTT assay showed a dose dependent increase in cytotoxic effect of ginger oleoresin against A549 lung cancer cell line. The IC₅₀ value was 20 μg/ml for the extract. Conclusion: The number of viable cells decreases as the drug concentration increases. Therefore, the present study concludes that ginger oleoresin possesses a strong cytotoxic effect. Hence, it is a promising candidate for anticancer therapy.