Chronic wounds place a heavy strain on patients and society.Diabetic woundsare a developing health concern that contribute significantly to the disability and mortality of diabetic patients. The study displayed the possible mechanisms by which 3D organo-hydrogel nanocomposite(PDA–TiO2@Ag) accelerate wound healing in diabetic rats. Streptozotocin (STZ) was used to induce diabetes through a singleI.P. injection. A fullthickness wound measuring 10mm was formed one week after induction of diabetes. Thirty adult male albino rats divided into: Normal control rats (not diabetic withnon-treated skin wounds),Diabetic control rats(diabetic with non-treated skin wounds), organo-hydrogel treated rats (diabeticwoundswere treated withorgano-hydrogelplacedtopically on the woundonce daily). Seven days after inducing the wound, samples from the wound treated by organo-hydrogelexhibitedmarked increase in the wound closure percentage as compared to normal and diabetic control groups. The finding was supported by histological analysis.Also, the gene expression of Bcl-2 Associated X-protein (BAX) and Insulin like growth factor (IGF) were assessed by qRT -PCR. The organo-hydrogel increased IGF levels and downregulated BAX levels. Hence, the organo-hydrogel can acceleratediabetic wound healing through reducing apoptosis and increasing the growth factors like IGF.