To study the genotoxic potentials of methanolic extracts of mimosa pud is against oral cancer cells. Oral cancer also called as mouth cancer is type of head and neck cancer and is cancerous tissue growth located in oral cavity It may arise as a primary lesion originating in any of the tissues in the mouth, by metastasis from a distant site of origin, or by extension from a neighboring anatomic structure, such as the nasal cavity. Alternatively, the oral cancers may originate in any of the tissues of the mouth, and may be of varied histologic types: teratoma,adenocarcinoma derived from a major or minor salivary gland, lymphoma from tonsillar or other lymphoid tissue, or melanoma from the pigment-producing cells of the oral mucosa. Cells were maintained in DMEM medium, supplemented with 10% Fetal Bovine Serum, at 37oC in a humidified atmosphere with 5% CO2. The cells were plated in 96 well flat bottom tissue culture plates at a density of approximately 1.2X 104 cells /well and allowed to attach overnight at 37oC. The medium was then discarded and cells were incubated with different concentrations of the samples (100, 200 & 300 µg) for 24 hours. The cell suspension was then collected and subjected to DNA isolation procedures. The methanolic extracts of M. pudica show anticancer activity on cancer activity on oral cancer cells. Here DNA fragmentation is done where more fragmentation is seen with dosage increased. So this proves that extracts show anticancer activity. Our study shows that the extracts which we used show anti cancer activity against oral cancer cells