Background: Nanotechnology has a major impact in a variety of applications in the biomedical, engineering, chemical, medicinal and food industries. Nanotechnology is employed to treat microbial infections. Nanotechnology is seen as an emerging scientific field in many aspects, and has a great impact on human daily life with various applications. Nutmeg oleoresin is used as a flavouring agent for foods. Nutmeg oleoresin has strong antimicrobial activity with less cytotoxic activity. Aim: The aim of the study was to analyse the antimicrobial activity of copper nanoparticles synthesized using nutmeg oleoresin Materials and Methods: The nutmeg oleoresin mediated copper nanoparticles was synthesized and confirmed by UV visible spectroscopy and antimicrobial activity was tested aganist staphylococcus aureus, Enterococcus faecalis and Streptococcus mutans and Candida albicans, by agar well diffusion method using respective media. Results: Nutmeg oleoresin mediated copper nanoparticles showed good results for antimicrobial activity. Among the four organisms, Candida albicans (fungal organism) showed more zones of inhibition when compared to Staphylococcus aureus, Streptococcus mutans and Enterococcus faecalis (bacterial organisms). Conclusion: Antimicrobial activity of copper nanoparticles prepared using nutmeg oleoresin is exhibited with significant antimicrobial activity with minimal side effects. Antimicrobial activity of copper nanoparticles showed a maximum zone of inhibition at 100μl.The sensitivity Candida albicans > Staphylococcus aureus> Streptococcus mutans > Enterococcus faecalis.