Background: Nanotechnology explores a lot of interesting approaches in the field of biomedicine. Researchers have recently developed interest in biomolecule mediated nanoparticle production because of its eco-friendly and non-toxic properties. Clova and cumin have long been used in traditional medicine to treat various illnesses. Aim: The purpose of this study is to use clove and cumin extract to produce the selenium nanoparticles via green approach. Materials And Method: Collection of clove and cumin extract then 1 g of clove and cumin, powder was dissolved in distilled water and boiled for 5–10 min at 60–70C. The solutions were then filtered using Whatman No. 1 filter paper. The filtered extract was collected, synthesis of selenium nanoparticles using an herbal formulation, and to finalise the antiinflammatory activity of silver nanoparticles. Results: It is one of the most effective methods in synthesis of selenium nanoparticles utilising green sources such as clove and cumin as antiinflammatory agents. The benefits are that they are nontoxic materials, requiring relatively less complicated and affordable equipment, biodegradable material processing, improved selectivity, and high yields. Conclusion: The findings of this study imply that selenium nanoparticles mediated by clove and cumin can be employed in biomedical applications in the future.