The aim of the study is to compare the hormonal levels of Luteinizing Hormone, Testosterone and 17-beta-estradiol in the blood of reproductively healthy cows with their levels in the blood of repeat breeder cows, in also to comparing the levels of minerals represented by (chloride, magnesium, potassium, phosphorous, sodium, Calcium, zinc and selenium) in cervical fluids of reproductively healthy cows with frequent estrus during estrus. The results of the hormonalstudy in the blood serum of the reproductively healthy cows showed that the levels of LH were 42.29 mIU/ml +-3.49 mIU/ml and testosterone was ng/ml 11.02 +- 0.54 and estradiol was 97.46 pg/ml +-4.14, while the hormonal levels of cows were Suffering from repeat breeder 60.55 mIU/ml +-2.86 pg/ml for LH, 14.86 +- 0.43 ng/ml for testosterone, and 149.9 +- 2.8 pg/ml for beta-estradiol -17, respectively. The results of the current study showed that the chloride concentration was 95.03 +- 1.38 mEq/l, the magnesium concentration was 0.305 +- 0.02 µg/dl, the potassium concentration was 4.36 +- 0.22 mEq/l, and the phosphorous concentration was 4.36 +- 0.22 mEq/l. It was 6.17 +-0.20 mg/dl, the sodium concentration was 120.8 +- 1.31 mEq/l, the calcium concentration was 10.73 +- 0.53 mg/dl, the zinc concentration was 15.23 +- 0.37 µg/dl, and the selenium concentration was 145.4 +- 0.85 µg/L. While their concentrations were 128.58 +- 0.86 mEq/l for chloride, 0.124 µg/dl +- 0.01 for magnesium, 6.66 mEq/l +- 0.25 for potassium, 4.35 mg/dl +- 0.18 for phosphorous, and 141.8 mEq/l +- 1.80 for sodium, 6.21 mg/dl +- 0.21 for calcium, 21.9 µg/dl +-0.99 for zinc, and 127.73 +- 2.57 µg/dl for selenium. There is also a Positive correlation between the levels of testosterone, beta-17-estradiol in the blood serum of cows and the concentrations of elements (chloride, potassium, sodium and zinc) in the estrous mucus of the study cows. The study also showed a positive correlation between the concentration of the element chloride and the concentration of (potassium, sodium and zinc) in the estrus mucus of the study cows.