Introduction: The sudden outbreak of the new coronavirus disease (Covid-19) may lead to job burnout due to this disease in paramedics and hospital guards. In this regard, the present study was conducted to evaluate and compare job burnout due to Covid-19 in paramedics and guards of educational hospitals of Semnan University of Medical Sciences in Semnan, Iran. Materials and Methods: In this cross-sectional descriptive-analytical study, 129 paramedics and guards of Kowsar and Amir Al-Momenin hospitals were assessed in terms of job burnout using the Maslach Burnout Inventory (1981). The collected data were analyzed using SPSS software. Results: In this study, it was shown that emotional exhaustion was more frequent in female paramedics and male guards with a positive history of Covid-19. Also, in terms of depersonalization, the staff of Kowsar hospital, especially the paramedics, had a higher score compared to Amir Al-Momenin Hospital. Also, a significant relationship was observed between the subscale of lack of personal success and the history of Covid-19 (P<0.05); In those who did not have a history of Covid-19, the level of this subscale was reported to be lower. Discussion: The results of the present study demonstrated that there is a significant relationship between job burnout and anxiety of coronavirus infection among paramedics and hospital guards; So that the mental and physical abilities of employees, especially in the health sector, have been affected by the outbreak of Covid-19. Therefore, more attention should be paid to hospital staff in these disease conditions