Introduction: Azadirachta indica (Neem) may be a rapid-growing plant even in severe drought which shed most of its leaves. The branches are wide and spreading. In this present investigation, we have prepared mouthwash using Stevia and Neem-based silver nanoparticles. Materials And Methods: Azadirachta indica and Stevia rebaudiana plant powder form was taken. 1g of Neem and Stevia extract powder mixed with 100ml of distilled water and then it was boiled, cooled and filtered with filtered paper and allowed to settle and the extract mixture was found to be 50ml and filtered. Results And Discussion :The plant had exhibited a potent cytotoxic effect at higher concentrations. Extracellular synthesis of silver nanoparticles using Neem and Stevia was primarily identified by visual identification; the appearance of silver and whitish colour in reaction mixture indicates nanoparticles. Conclusion :The present study revealed that the silver nanoparticles can be synthesised in a simple eco-friendly method using Neem and Stevia .