E-ISSN 2577-5669
 

Original Research 


Blood cellular, serum biochemical and organosomatic alterations in albino rats following sub-acute oral administration of varied doses of Pterocarpus santalinoides methanolic leaf extract.

Thelma Ebele Ihedioha, Isaac Uzoma Asuzu, Aruh Ottah Anaga, John Ikechukwu Ihedioha.

Abstract
Background: Leaf extracts of Pterocarpus santalinoides are used in ethno-medicine for the treatment of liver diseases. This study evaluated the blood cellular, serum biochemical and organosomatic alterations associated with sub-acute oral administration of Pterocarpus santalinoides methanol leaf extract (PSMLE) to albino rats.
Methods: Leaves of P. santalinoides used for the study were collected from the tree in June 2018. Twenty female rats, randomly assigned into four groups (1 4) of five each, were used for the study. Group 1 rats (untreated control) were given distilled water placebo, while groups 2, 3 and 4 were treated orally with 50, 250 and 500 mg/kg PSMLE, respectively. Treatment was done daily for 28 days, after which blood samples were collected for haematology and blood biochemistry. The rats were humanely sacrificed and vital body organs were eviscerated and weighed.
Results: Serum total protein levels of groups 2 and 3 rats were significantly (p<0.05) higher than that of groups 1 and 4, while serum globulin levels of group 2 was significantly higher (p<0.05) than that of groups 1 and 4. All other serum biochemical and all haematological parameters, and the relative liver, kidney, spleen and heart weights did not vary significantly (p>0.05) between the groups. Mean body weights of group 3 rats was significantly (p<0.05) higher than that of groups 1 and 2 on day 28.
Conclusion: Sub-acute administration of PSMLE to rats led to no significant haematological, serum biochemical and organosomatic alterations, except for higher serum total proteins and globulins, and higher body weights.

Key words: Blood biochemistry; Haematology; Organosomatic indices; Pterocarpus santalinoides extract; Sub-acute toxicity.


 
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How to Cite this Article
Pubmed Style

Ihedioha TE, Asuzu IU, Anaga AO, Ihedioha JI. Blood cellular, serum biochemical and organosomatic alterations in albino rats following sub-acute oral administration of varied doses of Pterocarpus santalinoides methanolic leaf extract.. J Complement Med Res. 2021; 12(1): 62-69. doi:10.5455/jcmr.2021.12.01.06


Web Style

Ihedioha TE, Asuzu IU, Anaga AO, Ihedioha JI. Blood cellular, serum biochemical and organosomatic alterations in albino rats following sub-acute oral administration of varied doses of Pterocarpus santalinoides methanolic leaf extract.. http://www.jocmr.com/?mno=129275 [Access: October 08, 2021]. doi:10.5455/jcmr.2021.12.01.06


AMA (American Medical Association) Style

Ihedioha TE, Asuzu IU, Anaga AO, Ihedioha JI. Blood cellular, serum biochemical and organosomatic alterations in albino rats following sub-acute oral administration of varied doses of Pterocarpus santalinoides methanolic leaf extract.. J Complement Med Res. 2021; 12(1): 62-69. doi:10.5455/jcmr.2021.12.01.06



Vancouver/ICMJE Style

Ihedioha TE, Asuzu IU, Anaga AO, Ihedioha JI. Blood cellular, serum biochemical and organosomatic alterations in albino rats following sub-acute oral administration of varied doses of Pterocarpus santalinoides methanolic leaf extract.. J Complement Med Res. (2021), [cited October 08, 2021]; 12(1): 62-69. doi:10.5455/jcmr.2021.12.01.06



Harvard Style

Ihedioha, T. E., Asuzu, . I. U., Anaga, . A. O. & Ihedioha, . J. I. (2021) Blood cellular, serum biochemical and organosomatic alterations in albino rats following sub-acute oral administration of varied doses of Pterocarpus santalinoides methanolic leaf extract.. J Complement Med Res, 12 (1), 62-69. doi:10.5455/jcmr.2021.12.01.06



Turabian Style

Ihedioha, Thelma Ebele, Isaac Uzoma Asuzu, Aruh Ottah Anaga, and John Ikechukwu Ihedioha. 2021. Blood cellular, serum biochemical and organosomatic alterations in albino rats following sub-acute oral administration of varied doses of Pterocarpus santalinoides methanolic leaf extract.. Journal of Complementary Medicine Research, 12 (1), 62-69. doi:10.5455/jcmr.2021.12.01.06



Chicago Style

Ihedioha, Thelma Ebele, Isaac Uzoma Asuzu, Aruh Ottah Anaga, and John Ikechukwu Ihedioha. "Blood cellular, serum biochemical and organosomatic alterations in albino rats following sub-acute oral administration of varied doses of Pterocarpus santalinoides methanolic leaf extract.." Journal of Complementary Medicine Research 12 (2021), 62-69. doi:10.5455/jcmr.2021.12.01.06



MLA (The Modern Language Association) Style

Ihedioha, Thelma Ebele, Isaac Uzoma Asuzu, Aruh Ottah Anaga, and John Ikechukwu Ihedioha. "Blood cellular, serum biochemical and organosomatic alterations in albino rats following sub-acute oral administration of varied doses of Pterocarpus santalinoides methanolic leaf extract.." Journal of Complementary Medicine Research 12.1 (2021), 62-69. Print. doi:10.5455/jcmr.2021.12.01.06



APA (American Psychological Association) Style

Ihedioha, T. E., Asuzu, . I. U., Anaga, . A. O. & Ihedioha, . J. I. (2021) Blood cellular, serum biochemical and organosomatic alterations in albino rats following sub-acute oral administration of varied doses of Pterocarpus santalinoides methanolic leaf extract.. Journal of Complementary Medicine Research, 12 (1), 62-69. doi:10.5455/jcmr.2021.12.01.06