AIM: The aim of the study was to prepare the herbal formulation of stevia and the piper longum nanoparticle and to evaluate its anti-inflammatory and cytotoxic effects. Materials and Methods: For the evaluation of antiinflammatory activity we used albumin denaturation assay and for the cytotoxic activity we used a brine shrimp lethality assay and evaluated the result according to the test results and the graph was made with the results Result : The nanoparticle of stevia and piper longum showed a marked anti inflammatory and cytotoxic activity with increased in their concentrations Conclusion: Stevia and piper longum nanoparticle markedly increase the anti inflammatory and cytotoxic activity compared to the standard and should be employed as a formulations